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Tissue culture responses of explants taken from branch sources with different degrees of juvenility in mature black locust (Robinia pseudoacacia) trees
Han KH, Shin DI, Keathley DE
TREE PHYSIOLOGY

17: (10) 671-675 OCT 1997


Abstract:
We studied the in vitro responses of cambial tissue and dormant vegetative buds obtained from top and epicormic branches of three mature black locust (Robinia pseudoacacia L.) trees. Cambial tissues isolated from epicormic branches produced more callus than cambial tissues isolated from top branches, whereas in vitro shoot cultures derived from buds excised from top branches grew faster than those derived from buds excised from epicormic branches. There were no significant differences between the two branch sources in in vitro bud break or shoot multiplication from bud explants or cambial-derived callus tissue, respectively. Furthermore, the top branches, generally considered to be the most mature in a tree, were not recalcitrant in terms of morphogenic capacity compared to epicormic branches.

Author Keywords:
black locust, cambial tissue culture, juvenility/maturity, shoot regeneration

KeyWords Plus:
QUERCUS-ROBUR L, HEDERA-HELIX L, ADULT, MICROPROPAGATION, REGENERATION, CHESTNUT, GROWTH

Addresses:
OREGON STATE UNIV, DEPT FOREST SCI, CORVALLIS, OR 97331.
HYUSUNG CATHOLIC UNIV, COLL NAT RESOURCES, DEPT PLANT BREEDING, HAYANG, KYUNGPOOK, SOUTH KOREA.
MICHIGAN STATE UNIV, DEPT FORESTRY, E LANSING, MI 48824.

Publisher:
HERON PUBLISHING, VICTORIA

IDS Number:
XX425

ISSN:
0829-318X


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