Matrix attachment regions (MARs) enhance transformation frequency and transgene expression in poplar -- Web of Science version 4.1 (ISI1)

Matrix attachment regions (MARs) enhance transformation frequency and transgene expression in poplar
Han KH, Ma CP, Strauss SH
TRANSGENIC RESEARCH

6: (6) 415-420 NOV 1997

Abstract:
We tested the value of a matrix attachment region (MAR) fragment derived from a tobacco gene for increasing the frequency of Agrobacterium-mediated transformation. A binary vector that carried a GUS reporter gene containing an intron and an nptII gene was modified to contain flanking MAR elements within the T-DNA borders. Vectors containing or lacking MARs were then used to transform tobacco, a readily transformable poplar clone (Populus tremula X P. alba), and a recalcitrant poplar clone (Populus trichocarpa x P. deltoides). MARs increased GUS gene expression approximately 10-fold in the two hybrid poplar clones and twofold in tobacco one month after cocultivation with Agrobacterium; MARs also increased the frequency of kanamycin-resistant poplar shoots recovered.

Author Keywords:
GUS, matrix attachment regions, Populus, transformation, transgene expression, Agrobacterium

KeyWords Plus:
REPORTER GENE-EXPRESSION, PLANT TRANSFORMATION, COPY NUMBER, POSITION, INACTIVATION, VARIABILITY, PROMOTERS, TOBACCO, MARKER, CELLS

Addresses:
OREGON STATE UNIV, DEPT FOREST SCI, CORVALLIS, OR 97331.

Publisher:
CHAPMAN HALL LTD, LONDON

IDS Number:
YK796

ISSN:
0962-8819

Article 54 of 179